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| Item Type: | Dataset |
|---|---|
| Title: | Dataset: rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome |
| Creators: |
Boehm, Volker  ORCID: https://orcid.org/0000-0001-7588-9842, Wallmeroth, Damaris ORCID: https://orcid.org/0000-0003-3437-4070, Wulf, Paul O. ORCID: https://orcid.org/0009-0002-6356-2361, Popp, Oliver ORCID: https://orcid.org/0000-0001-6240-4666, Teixeira Alves, Luiz Gustavo ORCID: https://orcid.org/0000-0002-5822-4168, Reinecke, Lucie ORCID: https://orcid.org/0009-0003-7083-0923, Riedel, Maximilian ORCID: https://orcid.org/0009-0002-9817-3083, Wyler, Emanuel ORCID: https://orcid.org/0000-0002-9884-1806, Franitza, Marek, Becker, Kerstin ORCID: https://orcid.org/0009-0009-7897-7181, Polkovnychenko, Karina ORCID: https://orcid.org/0009-0001-9468-3150, Del Giudice, Simone ORCID: https://orcid.org/0000-0003-0194-3767, Benlasfer, Nouhad, Mertins, Philipp ORCID: https://orcid.org/0000-0002-2245-528X, Landthaler, Markus ORCID: https://orcid.org/0000-0002-1075-8734 and Gehring, Niels H.
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| Abstract: | This dataset contains the imaging source data for the manuscript titled "Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated human transcriptome" by Boehm et al. published in Molecular Cell in 2025. The unprocessed and uncompressed imaging data (western blot and RT-PCR gels) for each main and supplemental figure can be found in the respective figure folder. For RT-PCR images, TIF (.tif) and raw Bio-Rad Image Lab Image Document (.scn) files are provided. For Western blots, multi-page TIFs (pages: 1. overlay, 2. raw chemiluminescence, 3. marker image, 4. final) are provided. Markers for western blots: PageRuler Prestained Protein Ladder (ThermoFisher Cat# 26617) and for RT-PCR: HyperLadder 1kb (Meridian Cat# BIO-33026). The PDF file "Boehm et al Overview" provides an overview of all main and supplemental figure images used in this study. |
| Source: | Mendeley Data |
| Publisher: | Elsevier |
| Date: | 31 August 2025 |
| Official Publication: | https://doi.org/10.17632/t9vzn5vwsz.1 |
| Related to: |
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