Generation of an NCS1 gene knockout human induced pluripotent stem cell line using CRISPR/Cas9

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Item Type:	Article
Title:	Generation of an NCS1 gene knockout human induced pluripotent stem cell line using CRISPR/Cas9
Creators Name:	Maierhof, S.K., Schinke, C., Cernoch, J., Hew, L., Bruske, L.P., Fernandez Vallone, V., Fischer, K., Stachelscheid, H., Huehnchen, P., Endres, M., Diecke, S., Telugu, N.S. and Boehmerle, W.
Abstract:	NCS1 (Neuronal calcium sensor protein 1) encodes a highly conserved calcium binding protein abundantly expressed in neurons. It modulates intracellular calcium homeostasis, calcium-dependent signaling pathways as well as neuronal transmission and plasticity. Here, we generated a NCS1 knockout human induced pluripotent stem cell (hiPSC) line using CRISPR-Cas9 genome editing. It shows regular expression of pluripotent markers, normal iPSC morphology and karyotype as well as no detectable off-target effects on top 6 potentially affected genes. This newly generated cell line constitutes a valuable tool for studying the role of NCS1 in the pathophysiology of various neuropsychiatric disorders and non-neurological disease.
Keywords:	Calcium, CRISPR-Cas Systems, Gene Editing, Gene Knockout Techniques, Induced Pluripotent Stem Cells
Source:	Stem Cell Research
ISSN:	1873-5061
Publisher:	Elsevier
Volume:	73
Page Range:	103253
Date:	December 2023
Official Publication:	https://doi.org/10.1016/j.scr.2023.10325
PubMed:	View item in PubMed

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