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| Item Type: | Article |
|---|---|
| Title: | Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae |
| Creators Name: | Corradi, L. and Zaupa, M. and Sawamiphak, S. and Filosa, A. |
| Abstract: | The larval zebrafish has emerged as a very useful model organism to study the neuronal circuits controlling neuroendocrine and behavioral responses to stress. This protocol describes how to expose zebrafish larvae to hyperosmotic stress and test whether candidate populations of neurons are activated or inhibited by the stressor using a relatively rapid immunofluorescence staining approach. This approach takes advantage of the phosphorylation of the extracellular signal-regulated kinase (ERK) upon neuronal activation. For complete details on the use and execution of this protocol, please refer to Corradi et al. (2022). |
| Keywords: | Microscopy, Model Organisms, Neuroscience Behavior, Animals, Zebrafish |
| Source: | STAR Protocols |
| ISSN: | 2666-1667 |
| Publisher: | Cell Press / Elsevier |
| Volume: | 3 |
| Number: | 4 |
| Page Range: | 101731 |
| Date: | 16 December 2022 |
| Official Publication: | https://doi.org/10.1016/j.xpro.2022.101731 |
| PubMed: | View item in PubMed |
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