Item Type: | Article |
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Title: | Generation of redirected engineered human chimeric antigen receptor (CAR) T cells |
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Creators Name: | Bunse, M. and Höpken, U.E. |
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Abstract: | Chimeric antigen receptor (CAR) T cell therapy that involves genetic engineering a patient's own immune cells with antigen-specific receptors has shown remarkable efficacy in blood cancer treatment. Numerous clinical studies with CAR T cells targeting the blood cell surface protein CD19 led to the FDA 's first approval of a genetically engineered cell therapy. The process of generating potent CAR T cells involves several carefully performed manufacturing steps. Here, we describe the generation of redirected engineered human CAR T cells for preclinical studies starting with the CAR design, retroviral gene transfer, detection of CAR expression, and expansion of transduced T cells. |
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Keywords: | Chimeric Antigen Receptor, T Cells, Retroviral Transduction, Tumor Immunotherapy |
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Source: | Methods in Molecular Biology |
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Series Name: | Methods in Molecular Biology |
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Title of Book: | Gene therapy of cancer : methods and protocols |
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ISSN: | 1064-3745 |
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ISBN: | 9781071624401 |
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Publisher: | Springer / Humana Press |
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Volume: | 2521 |
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Number: | 3 |
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Page Range: | 67-83 |
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Number of Pages: | 0 |
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Date: | 23 June 2022 |
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Official Publication: | https://doi.org/10.1007/978-1-0716-2441-8_4 |
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PubMed: | View item in PubMed |
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Related to: | |
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