Chromatin-sensitive cryptic promoters encode alternative protein isoforms in yeast

Item Type:

Preprint

Title:

Creators Name:

Wei, W. and Hennig, B.P. and Wang, J. and Zhang, Y. and Piazza, I. and Sanchez, Y.P. and Chabbert, C.D. and Adjalley, S.H. and Steinmetz, L.M. and Pelechano, V.

Abstract:

Cryptic transcription is widespread and generates a heterogeneous group of RNA molecules of unknown function. To improve our understanding of cryptic transcription, we investigated their transcription start site usage, chromatin organization and post-transcriptional consequences in Saccharomyces cerevisiae. We show that transcription start sites (TSSs) of chromatin-sensitive internal cryptic transcripts retain comparable features of canonical TSSs in terms of DNA sequence, directionality and chromatin accessibility. We degine the 5’ and 3’ boundaries of cryptic transcripts and show that, contrary to RNA degradation-sensitive ones, they often overlap with the end of the gene thereby using the canonical polyadenylation site and associate to polyribosomes. We show that chromatin-sensitive cryptic transcripts can be recognized by ribosomes and may produce truncated polypeptides from downstream, in-frame start codons. Finally, we congirm the presence of the predicted polypeptides by reanalyzing N-terminal proteomic datasets. Our work suggests that a fraction of chromatin-sensitive internal cryptic promoters are in fact alternative truncated mRNA isoforms. The expression of these chromatin-sensitive isoforms is conserved from yeast to human expanding the functional consequences of cryptic transcription and proteome complexity.

Source:

bioRxiv

Title of Book:

Chromatin-sensitive cryptic promoters encode alternative protein isoforms in yeast

ISSN:

1664-3224

Publisher:

Cold Spring Harbor Laboratory Press

Article Number:

403543

Date:

12 August 2019

Official Publication:

https://doi.org/10.1101/403543

Related to:

URL	URL Type
https://edoc.mdc-berlin.de/18847/	Final version

Repository Staff Only: item control page