Label-free detection of global morphology changes in confluent cell layers utilizing quantitative phase imaging with digital holographic microscopy

Item Type:	Conference or Workshop Item
Title:	Label-free detection of global morphology changes in confluent cell layers utilizing quantitative phase imaging with digital holographic microscopy
Creators Name:	Kemper, B. and Pohl, L. and Kaiser, M. and Döpker, E. and Schnekenburger, J. and Ketelhut, S.
Abstract:	For the example of digital holographic microscopy (DHM), a variant of quantitative phase imaging (QPI), we explored strategies for the analysis of confluent cell layers utilizing histogram based-evaluation of quantitative phase images. The applicability of the proposed numerical evaluation procedures is illustrated by the DHM-based quantification of drug induced cell morphology changes. Our results show that histogram-based evaluation of quantitative phase images enables a highly reliable quantitative observation of global cellular morphology changes in confluent cell layers.
Keywords:	Quantitative Phase Imaging, Digital Holographic Microscopy, Label-Free Live Cell Imaging, Confluent Cell Layers
Source:	Proceedings of SPIE
Title of Book:	Advances in Microscopic Imaging II
ISSN:	0277-786X
Publisher:	International Society for Optics and Photonics (SPIE)
Volume:	11076
Page Range:	30
Date:	22 July 2019
Official Publication:	https://doi.org/10.1117/12.2527189

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