Low-density granulocytes are a novel immunopathological feature in both multiple sclerosis and neuromyelitis optica spectrum disorder

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Item Type:

Preprint

Title:

Low-density granulocytes are a novel immunopathological feature in both multiple sclerosis and neuromyelitis optica spectrum disorder

Creators Name:

Ostendorf, L. and Mothes, R. and van Koppen, S. and Lindquist, R.L. and Bellmann-Strobl, J. and Asseyer, S. and Ruprecht, K. and Alexander, T. and Niesner, R.A. and Hauser, A.E. and Paul, F. and Radbruch, H.

Abstract:

Objective: To investigate whether low-density granulocytes (LDGs) are a immunophenotypic feature of patients with multiple sclerosis (MS) and/or neuromyelitis optica spectrum disorder (NMOSD). Methods: Blood samples were collected from 26 patients with NMOSD and 20 patients with MS, as well as from 18 patients with Systemic Lupus Erythematosus (SLE) and 23 Healthy Donors (HD). We isolated peripheral blood mononuclear cells (PBMCs) with density gradient separation and stained the cells with antibodies against CD14, CD15, CD16, and CD45, and analysed the cells by flow cytometry or imaging flow cytometry. We defined LDGs as CD14-CD15high and calculated their share in total PBMC leukocytes (CD45+) as well as the share of CD16hi LDGs. Clinical data on disease course, medication, and antibody status were obtained. Results: LDGs were significantly more common in MS and NMOSD than in HDs, comparable to SLE samples (median values HD 0.2%, MS 0.9%, NMOSD 2.1%, SLE 4.3%). 0/23 of the HDs, but 17/20 NMOSD and 11/17 MS samples as well as 13/15 SLE samples had at least 0.7 % LDGs. NMOSD patients without continuous immunosuppressive treatment had significantly more LDGs compared to their treated counterparts. LDG nuclear morphology ranged from segmented to rounded, suggesting a heterogeneity within the group. Conclusion: LDGs are a feature of the immunophenotype in some patients with MS and NMOSD.

Source:

bioRxiv

ISSN:

1664-3224

Publisher:

Cold Spring Harbor Laboratory Press

Volume:

Page Range:

2725

Article Number:

668160

Date:

12 June 2019

Official Publication:

https://doi.org/10.1101/668160

Related to:

URL	URL Type
https://edoc.mdc-berlin.de/18607/	Final version

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