Nanocapsule induced morphology and migration changes in single cell layers quantified with digital holographic microscopy

Item Type:	Conference or Workshop Item
Title:	Nanocapsule induced morphology and migration changes in single cell layers quantified with digital holographic microscopy
Creators Name:	Kaiser, M. and Pohl, L. and Ketelhut, S. and Kastl, L. and Gorzelanny, C. and Götte, M. and Schnekenburger, J. and Goycoolea, F.M. and Kemper, B.
Abstract:	We applied quantitative phase imaging (QPI) for quantification of nanocapsule-induced morphology and migration changes in single cell layers. In time-lapse observations, cells were monitored with a Mach-Zehnder interferometerbased off-axis digital holographic microscopy (DHM) setup. For quantification of cell migration, single cells were tracked in the recorded series of quantitative DHM phase images. Moreover, QPI images were evaluated as novel stainfree assay to quantify the temporal course of global cellular morphology changes. The label-free acquired data shows that capsaicin-loaded and unloaded chitosan nanocapsules, and also free capsaicin, significantly influence the direction of cell migration and cellular motility.
Keywords:	Quantitative Phase Imaging, Digital Holographic Microscopy, Label-Free, Cell Migration, Cell Morphology, Nanosystems, Drug Delivery
Source:	Proceedings of SPIE
Series Name:	Proceedings of SPIE
Title of Book:	Quantitative Phase Imaging V
ISSN:	0277-786X
Publisher:	International Society for Optics and Photonics (SPIE)
Volume:	10887
Page Range:	108871F
Date:	4 March 2019
Official Publication:	https://doi.org/10.1117/12.2509214

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