Spatial transcriptomics of C. elegans males and hermaphrodites identifies sex-specific differences in gene expression patterns
Item Type: | Article |
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Title: | Spatial transcriptomics of C. elegans males and hermaphrodites identifies sex-specific differences in gene expression patterns |
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Creators Name: | Ebbing, A. and Vértesy, Á. and Betist, M.C. and Spanjaard, B. and Junker, J.P. and Berezikov, E. and van Oudenaarden, A. and Korswagen, H.C. |
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Abstract: | To advance our understanding of the genetic programs that drive cell and tissue specialization, it is necessary to obtain a comprehensive overview of gene expression patterns. Here, we have used spatial transcriptomics to generate high-resolution, anteroposterior gene expression maps of C. elegans males and hermaphrodites. To explore these maps, we have developed computational methods for discovering region- and tissue-specific genes. We have found extensive sex-specific gene expression differences in the germline and sperm and discovered genes that are specifically expressed in the male reproductive tract. These include a group of uncharacterized genes that encode small secreted proteins that are required for male fertility. We conclude that spatial gene expression maps provide a powerful resource for identifying tissue-specific gene functions in C. elegans. Importantly, we found that expression maps from different animals can be precisely aligned, enabling transcriptome-wide comparisons of gene expression patterns. |
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Keywords: | C. elegans, Spatial Transcriptomics, mRNA Sequencing, CEL-Seq, Germline, Sperm, Male Fertility, Animals, Caenorhabditis elegans |
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Source: | Developmental Cell |
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ISSN: | 1534-5807 |
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Publisher: | Cell Press |
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Volume: | 47 |
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Number: | 6 |
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Page Range: | 801-813 |
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Date: | 17 December 2018 |
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Official Publication: | https://doi.org/10.1016/j.devcel.2018.10.016 |
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PubMed: | View item in PubMed |
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Related to: | |
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