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| Item Type: | Article |
|---|---|
| Title: | Characterization of transcription termination-associated RNAs: new insights into their biogenesis, tailing, and expression in primary tumors |
| Creators Name: | Laudadio, I. and Formichetti, S. and Gioiosa, S. and Klironomos, F. and Rajewsky, N. and Macino, G. and Carissimi, C. and Fulci, V. |
| Abstract: | Next-generation sequencing has uncovered novel classes of small RNAs (sRNAs) in eukaryotes, in addition to the well-known miRNAs, siRNAs, and piRNAs. In particular, sRNA species arise from transcription start sites (TSSs) and the transcription termination sites (TTSs) of genes. However, a detailed characterization of these new classes of sRNAs is still lacking. Here, we present a comprehensive study of sRNAs derived from TTSs of expressed genes (TTSa-RNAs) in human cell lines and primary tissues. Taking advantage of sRNA-sequencing, we show that TTSa-RNAs are present in the nuclei of human cells, are loaded onto both AGO1 and AGO2, and their biogenesis does not require DICER and AGO2 endonucleolytic activity. TTSa-RNAs display a strong bias against a G residue in the first position at 5 end, a known feature of AGO-bound sRNAs, and a peculiar oligoA tail at 3 end. AGO-bound TTSa-RNAs derive from genes involved in cell cycle progression regulation and DNA integrity checkpoints. Finally, we provide evidence that TTSa-RNAs can be detected by sRNA-Seq in primary human tissue, and their expression increases in tumor samples as compared to nontumor tissues, suggesting that in the future, TTSa-RNAs might be explored as biomarker for diagnosis or prognosis of human malignancies. |
| Keywords: | Noncoding RNAs, Argonaute Proteins, Start Sites, Disease, Dicer, Recognition, microRNAs, Framework, Elements, Complex |
| Source: | International Journal of Genomics |
| ISSN: | 2314-436X |
| Publisher: | Hindawi |
| Volume: | 2018 |
| Page Range: | 1243858 |
| Date: | 26 April 2018 |
| Official Publication: | https://doi.org/10.1155/2018/1243858 |
| PubMed: | View item in PubMed |
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