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| Item Type: | Article |
|---|---|
| Title: | Zebrafish enhancer detection (ZED) vector: a new tool to facilitate transgenesis and the functional analysis of cis-regulatory regions in zebrafish |
| Creators Name: | Bessa, J., Tena, J.J., de la Calle-Mustienes, E., Fernandez-Minan, A., Naranjo, S., Fernandez, A., Montoliu, L., Akalin, A., Lenhard, B., Casares, F. and Gomez-Skarmeta, J.L. |
| Abstract: | The identification and characterization of the regulatory activity of genomic sequences is crucial for understanding how the information contained in genomes is translated into cellular function. The cis-regulatory sequences control when, where, and how much genes are transcribed and can activate (enhancers) or repress (silencers) gene expression. Here, we describe a novel Tol2 transposon-based vector for assessing enhancer activity in the zebrafish (Danio rerio). This Zebrafish Enhancer Detector (ZED) vector harbors several key improvements, among them a sensitive and specific minimal promoter chosen for optimal enhancer activity detection, insulator sequences to shield the minimal promoter from position effects, and a positive control for transgenesis. Additionally, we demonstrate that highly conserved noncoding sequences homologous between humans and zebrafish largely with enhancer activity largely retain their tissue-specific enhancer activity during vertebrate evolution. More strikingly, insulator sequences from mouse and chicken, but not conserved in zebrafish, maintain their insulator capacity when tested in this model. |
| Keywords: | Enhancer Detection, Gene Regulation, Insulators, Transgenesis, Animals, Mice, Zebrafish |
| Source: | Developmental Dynamics |
| ISSN: | 1058-8388 |
| Publisher: | Wiley-Liss |
| Volume: | 238 |
| Number: | 9 |
| Page Range: | 2409-2417 |
| Date: | September 2009 |
| Official Publication: | https://doi.org/10.1002/dvdy.22051 |
| PubMed: | View item in PubMed |
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