Suspension medium influences interaction of mesenchymal stromal cells with endothelium and pulmonary toxicity after transplantation in mice

Item Type:	Article
Title:	Suspension medium influences interaction of mesenchymal stromal cells with endothelium and pulmonary toxicity after transplantation in mice
Creators Name:	Deak, E. and Ruester, B. and Keller, L. and Eckert, K. and Fichtner, I. and Seifried, E. and Henschler, R.
Abstract:	Intravenous (i.v.) transplantation and subsequent homing of Mesenchymal Stromal Cells (MSC) may be adversely influenced by their relatively high adhesion capacity and their tendency to aggregate, leading to clogging of capillaries especially in the lungs. We evaluated the ability of murine MSC suspended in EDTA or heparin in buffered saline solution on their spontaneous adhesion to endothelial cells in vitro, under shear stress and their in vivo tolerability after i.v. injection. We show that suspension of MSC in heparin was highly beneficial, avoiding clinical symptoms in 95% of mice, whereas application of MSC suspended in PBS/EDTA or control buffer caused severe pulmonary reactions and partly, death. In vitro studies using parallel plate flow chambers revealed increased adhesion of MSC suspended in PBS/EDTA to endothelial cells compared with MSC in PBS/heparin. These data provide a means to predict and to interfere with toxicity of i.v. transplanted MSC.
Keywords:	Buffers, Cell Aggregation, Cell Communication, Culture Media, Endothelial Cells, Intravenous Injections, Lung, Mesenchymal Stem Cell Transplantation, Mechanical Stress, Stromal Cells, Suspensions, Animals, Mice
Source:	Cytotherapy
ISSN:	1465-3249
Publisher:	Taylor & Francis
Volume:	12
Number:	2
Page Range:	260-264
Date:	April 2010
Official Publication:	https://doi.org/10.3109/14653240903401840
PubMed:	View item in PubMed

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