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Frameshift mutations in coding repeats of protein tyrosine phosphatase genes in colorectal tumors with microsatellite instability

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Item Type:Article
Title:Frameshift mutations in coding repeats of protein tyrosine phosphatase genes in colorectal tumors with microsatellite instability
Creators Name:Korff, S. and Woerner, S.M. and Yuan, Y.P. and Bork, P. and von Knebel Doeberitz, M. and Gebert, J.
Abstract:BACKGROUND: Protein tyrosine phosphatases (PTPs) like their antagonizing protein tyrosine kinases are key regulators of signal transduction thereby assuring normal control of cellular growth and differentiation. Increasing evidence suggests that mutations in PTP genes are associated with human malignancies. For example, mutational analysis of the tyrosine phosphatase (PTP) gene superfamily uncovered genetic alterations in about 26 % of colorectal tumors. Since in these studies tumors have not been stratified according to genetic instability status we hypothesized that colorectal tumors characterized by high-level of microsatellite instability (MSI-H) might show an increased frequency of frameshift mutations in those PTP genes that harbor long mononucleotide repeats in their coding region (cMNR). RESULTS: Using bioinformatic analysis we identified 16 PTP candidate genes with long cMNRs that were examined for genetic alterations in 19 MSI-H colon cell lines, 54 MSI-H colorectal cancers, and 17 MSI-H colorectal adenomas. Frameshift mutations were identified only in 6 PTP genes, of which PTPN21 show the highest mutation frequency at all in MSI-H tumors (17 %). CONCLUSIONS: Although about 32 % of MSI-H tumors showed at least one affected PTP gene, and cMNR mutation rates in PTPN21, PTPRS, and PTPN5 are higher than the mean mutation frequency of MNRs of the same length, mutations within PTP genes do not seem to play a common role in MSI tumorigenesis, since no cMNR mutation frequency reached statistical significance and therefore, failed prediction as a Positive Selective Target Gene.
Keywords:Cell Line, Neoplastic Cell Transformation, Colorectal Neoplasms, Computational Biology, DNA Mutational Analysis, Frameshift Mutation, Microsatellite Instability, Microsatellite Repeats, Open Reading Frames, Protein Tyrosine Phosphatases
Source:BMC Cancer
ISSN:1471-2407
Publisher:BioMed Central (U.K.)
Volume:8
Number:1
Page Range:329
Date:10 November 2008
Official Publication:https://doi.org/10.1186/1471-2407-8-329
PubMed:View item in PubMed

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