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Distinct isocomplexes of the TRAPP trafficking factor coexist inside human cells

Item Type:Article
Title:Distinct isocomplexes of the TRAPP trafficking factor coexist inside human cells
Creators Name:Kuemmel, D. and Oeckinghaus, A. and Wang, C. and Krappmann, D. and Heinemann, U.
Abstract:The transport protein particle (TRAPP) complex is required for proper vesicular transport from the ER to the Golgi. The composition of yeast TRAPP is well characterized, but the organization of mammalian TRAPP complex remains elusive. Using a tandem affinity purification (TAP) approach, we provide first experimental proof for the association of NIBP (NIK/IKKbeta binding protein) with Bet3 and find two human paralogs of Trs33 (A and B) associated with Bet3. Interaction studies and gel filtration analysis reveal that both proteins are part of human TRAPP and might mark two distinct isocomplexes that exert different functions in the regulation of ER-to-Golgi traffic.
Keywords:Vesicular Transport, TRAPP, Golgi, Bet3, Trs33
Source:FEBS Letters
ISSN:0014-5793
Publisher:Elsevier (The Netherlands)
Volume:582
Number:27
Page Range:3729-3733
Date:12 November 2008
Additional Information:The original publication is available at www.sciencedirect.com
Official Publication:https://doi.org/10.1016/j.febslet.2008.09.056
PubMed:View item in PubMed

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