Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Large gene overlaps in prokaryotic genomes: result of functional constraints or mispredictions?

[img] PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
602kB

Item Type:Article
Title:Large gene overlaps in prokaryotic genomes: result of functional constraints or mispredictions?
Creators Name:Palleja, A. and Harrington, E.D. and Bork, P.
Abstract:BACKGROUND: Across the fully sequenced microbial genomes there are thousands of examples of overlapping genes. Many of these are only a few nucleotides long and are thought to function by permitting the coordinated regulation of gene expression. However, there should also be selective pressure against long overlaps, as the existence of overlapping reading frames increases the risk of deleterious mutations. Here we examine the longest overlaps and assess whether they are the product of special functional constraints or of erroneous annotation. RESULTS: We analysed the genes that overlap by 60 bps or more among 338 fully-sequenced prokaryotic genomes. The likely functional significance of an overlap was determined by comparing each of the genes to its respective orthologs. If a gene showed a significantly different length from its orthologs it was considered unlikely to be functional and therefore the result of an error either in sequencing or gene prediction. Focusing on 715 co-directional overlaps longer than 60 bps, we classified the erroneous ones into five categories: i) 5'-end extension of the downstream gene due to either a mispredicted start codon or a frameshift at 5'-end of the gene (409 overlaps), ii) fragmentation of a gene caused by a frameshift (163), iii) 3'-end extension of the upstream gene due to either a frameshift at 3'-end of a gene or point mutation at the stop codon (68), iv) Redundant gene predictions (4), v) 5' & 3'-end extension which is a combination of i) and iii) (71). We also studied 75 divergent overlaps that could be classified as misannotations of group i). Nevertheless we found some convergent long overlaps (54) that might be true overlaps, although an important part of convergent overlaps could be classified as group iii) (124). CONCLUSIONS: Among the 968 overlaps larger than 60 bps which we analysed, we did not find a single real one among the co-directional and divergent orientations and concluded that there had been an excessive number of misannotations. Only convergent orientation seems to permit some long overlaps, although convergent overlaps are also hampered by misannotations. We propose a simple rule to flag these erroneous gene length predictions to facilitate automatic annotation.
Keywords:Amino Acid Sequence, Base Sequence, Initiator Codon, Terminator Codon, Factual Databases, Molecular Evolution, Frameshift Mutation, Overlapping Genes, Genome, Molecular Sequence Data, Open Reading Frames, Prokaryotic Cells, Amino Acid Sequence Homology
Source:BMC Genomics
ISSN:1471-2164
Publisher:BioMed Central (U.K.)
Volume:9
Number:1
Page Range:335
Date:15 July 2008
Official Publication:https://doi.org/10.1186/1471-2164-9-335
PubMed:View item in PubMed

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library