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Binding of galectin-1 (gal-1) to the Thomsen-Friedenreich (TF) antigen on trophoblast cells and inhibition of proliferation of trophoblast tumor cells in vitro by gal-1 or an anti-TF antibody

Item Type:Article
Title:Binding of galectin-1 (gal-1) to the Thomsen-Friedenreich (TF) antigen on trophoblast cells and inhibition of proliferation of trophoblast tumor cells in vitro by gal-1 or an anti-TF antibody
Creators Name:Jeschke, U. and Karsten, U. and Wiest, I. and Schulze, S. and Kuhn, C. and Friese, K. and Walzel, H.
Abstract:Galectin-1 (gal-1), a member of the mammalian beta-galactoside-binding proteins, recognizes preferentially Galbeta1-4GlcNAc sequences of several cell surface oligosaccharides. We demonstrate histochemically that the lectin recognizes appropriate glycotopes on the syncytiotrophoblast and extravillous trophoblast layer from second trimester human placenta and on BeWo chorion carcinoma cells. Gal-1 binding to BeWo cells was diminished by the Thomsen-Friedreich (TF)-disaccharide (Galbeta1-3GalNAc-) conjugated to polyacrylamide (TF-PAA). Gal-1 also inhibited BeWo cell proliferation in a concentration-dependent manner. Similar antiproliferative effects were also observed with an anti-TF monoclonal antibody (mAb, A78-G/A7). Therefore, we conclude that ligation of Galbeta1-4GlcNAc and Galbeta1-3GalNAc epitopes on BeWo cells may have regulatory effects on cell proliferation.
Keywords:Galectin-1, Thomsen-Friedenreich, Immunocytochemistry, Placenta, BeWo cells
Source:Histochemistry and Cell Biology
ISSN:0948-6143
Publisher:Springer (Germany)
Volume:126
Number:4
Page Range:437-444
Date:October 2006
Official Publication:https://doi.org/10.1007/s00418-006-0178-1
PubMed:View item in PubMed

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