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A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays

Item Type:Article
Title:A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays
Creators Name:Hampf, M. and Gossen, M.
Abstract:We established a quantitative reporter gene protocol, the P/Rluc assay system, allowing the sequential measurement of Photinus and Renilla luciferase activities from the same extract. Other than comparable commercial reporter assay systems and their noncommercial counterparts, the P/Rluc assay system was formulated under the aspect of full compatibility with standard methods for protein assays. This feature greatly expands the range of applications for assay systems quantifying the expression of multiple luciferase reporters.
Keywords:Photinus Luciferase, Renilla Luciferase, Dual-Luciferase, Protein Assay, Firefly, Animals, Fireflies
Source:Analytical Biochemistry
ISSN:0003-2697
Publisher:Academic Press (U.S.A.)
Volume:356
Number:1
Page Range:94-99
Date:1 September 2006
Official Publication:https://doi.org/10.1016/j.ab.2006.04.046
PubMed:View item in PubMed

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