Stable chromosomal units determine the spatial and temporal organization of DNA replication

Tools

Item Type:	Article
Title:	Stable chromosomal units determine the spatial and temporal organization of DNA replication
Creators Name:	Sadoni, N., Cardoso, M.C., Stelzer, E.H., Leonhardt, H. and Zink, D.
Abstract:	DNA replication occurs in mammalian cells at so-called replication foci occupying defined nuclear sites at specific times during S phase. It is an unresolved problem how this specific spatiotemporal organization of replication foci is determined. Another unresolved question remains as to what extent DNA is redistributed during S phase. To investigate these problems, we visualized the replicating DNA and the replication machinery simultaneously in living HeLa cells. Time-lapse analyses revealed that DNA was not redistributed to other nuclear sites during S phase. Furthermore, the results showed that DNA is organized into stable aggregates equivalent to replication foci. These aggregates, which we call sub-chromosomal foci, stably maintained their replication timing from S phase to S phase. During S-phase progression, the replication machinery sequentially proceeded through spatially adjacent sets of sub-chromosomal foci. These findings imply that the specific nuclear substructure of chromosomes and the order of their stable subunits determine the spatiotemporal organization of DNA replication.
Keywords:	Replication Dynamics, Replication Foci, Sub-Chromosomal Foci, Animals, Drosophila
Source:	Journal of Cell Science
ISSN:	0021-9533
Publisher:	Company of Biologists
Volume:	117
Number:	22
Page Range:	5353-5365
Date:	15 October 2004
Official Publication:	https://doi.org/10.1242/jcs.01412
PubMed:	View item in PubMed

Repository Staff Only: item control page