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| Item Type: | Article |
|---|---|
| Title: | RNA-containing adenovirus/polyethylenimine transfer complexes effectively transduce dendritic cells and induce antigen-specific T cell responses |
| Creators Name: | Gust, T.C. and Diebold, S.S. and Cotten, M. and Zenke, M. |
| Abstract: | Background. Dendritic cells (DCs) are the most potent antigen-presenting cells in initiating primary immune responses. Given the unique properties of DCs, gene-modified DCs represent a particularly attractive approach for immunotherapy of diseases such as cancer. Methods. Gene-modified DCs were obtained by a receptor-mediated gene delivery system using adenovirus (Ad) particles as ligand and RNA or DNA condensed by polyethylenimine (PEI). In vitro transcribed polyadenylated or non-polyadenylated RNA was used. RNA-transduced DCs were generated expressing chicken ovalbumin (OVA) or chimeric constructs thereof, and compared with DNA-transduced DCs. Results. Ad/PEI transfection complexes efficiently delivered RNA into DCs. Such RNA-transduced DCs induced OVA-specific T cell responses more effectively than DNA-transduced DCs. Furthermore, DCs transduced with polyadenylated RNA were more potent in stimulating CD4+ and CD8+ T cell responses than DCs transduced with non-polyadenylated RNA and this was particularly important for CD4+ T cell responses. Conclusions. Ad/PEI/RNA transfection is an efficient means for generating RNA-transduced DCs and for stimulating antigen-specific T cell responses. Polyadenylation of RNA enhances CD8+ T cell responses and is essential for CD4+ T cell responses. |
| Keywords: | Ad/PEI Transfection, Dendritic Cells, Gene Delivery, Ovalbumin, T Cell Activation, Animals, Mice |
| Source: | Journal of Gene Medicine |
| ISSN: | 1099-498X |
| Publisher: | John Wiley & Sons |
| Volume: | 6 |
| Number: | 4 |
| Page Range: | 464-470 |
| Date: | 1 April 2004 |
| Official Publication: | https://doi.org/10.1002/jgm.492 |
| PubMed: | View item in PubMed |
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