Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

A new approach for the treatment of malignant melanoma: Enhanced antitumor efficacy of an albumin-binding doxorubicin prodrug that is cleaved by matrix metalloproteinase 2

Item Type:Article
Title:A new approach for the treatment of malignant melanoma: Enhanced antitumor efficacy of an albumin-binding doxorubicin prodrug that is cleaved by matrix metalloproteinase 2
Creators Name:Mansour, A.M. and Drevs, J. and Esser, N. and Hamada, F.M. and Badary, O.A. and Unger, C. and Fichtner, I. and Kratz, F.
Abstract:The progression of malignant melanoma is characterized by overexpression of a number of matrix metalloproteinases (MMPs), especially MMP-2, which play a critical role in the degradation of basement membranes and the extracellular matrix. Consequently, we assessed a drug targeting strategy in which the protease activity of MMP-2 is exploited to release an anticancer agent from a macromolecular carrier, i.e., circulating albumin. For this purpose, a water-soluble maleimide derivative of doxorubicin (1) incorporating a MMP-2 specific peptide sequence (Gly-Pro-Leu-Gly-Ile-Ala-Gly-Gln) was developed that binds rapidly and selectively to the cysteine-34 position of circulating albumin. The albumin-bound form of 1 was efficiently and specifically cleaved by MMP-2 liberating a doxorubicin tetrapeptide (Ile-Ala-Gly-Gln-DOXO) and subsequently doxorubicin. In vivo, 1 was superior to the parent compound doxorubicin in the A375 human melanoma xenograft, which is characterized by a high expression of MMP-2.
Keywords:Albumins, Antineoplastic Antibiotics, Cultured Tumor Cells, Doxorubicin, Maleimides, Matrix Metalloproteinase 2, Melanoma, Oligopeptides, Prodrugs
Source:Cancer Research
ISSN:0008-5472
Publisher:American Association for Cancer Research (U.S.A.)
Volume:63
Number:14
Page Range:4062-4066
Date:15 July 2003
Official Publication:http://cancerres.aacrjournals.org/cgi/content/abstract/63/14/4062
PubMed:View item in PubMed

Repository Staff Only: item control page

Open Access
MDC Library