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An immortalized cell line expresses properties of activated microglial cells

Item Type:Article
Title:An immortalized cell line expresses properties of activated microglial cells
Creators Name:Bocchini, V. and Mazzolla, R. and Barluzzi, R. and Blasi, E. and Sick, P. and Kettenmann, H.
Abstract:Murine cultured microglial cells were immortalized after infection with a v-raf/v-myc recombinant retrovirus. This immortalized cell line (BV-2) shares properties with body macrophages with respect to the antigen profile, their phagocytic capacity and antimicrobial activity. BV-2 cells are not constitutively able to kill tumor cells in vitro, but acquire antitumor activity following an increase in [Ca++]i. BV-2 cells, like microglial cells, are however, distinct from peripheral macrophages by their expression of inwardly rectifying K+ channels in concert with a lack in outwardly rectifying K+ channels and the formation of spineous processes. The BV-2 cell line thus represents a suitable model for in vitro studies of activated microglial cells.
Keywords:Aspergillus Fumigatus, Candida Albicans, Cell Line, Transformed Cell Line, Central Nervous System, Colony-Forming Units Assay, Cryptococcus Neoformans, Electrophysiology, Myc Genes, Interleukin-1, Macrophages, Membrane Potentials, Mesoderm, Muramidase, Oncogene Proteins v-raf, Oncogenes, Phagocytosis, Protein-Tyrosine Kinases, Oncogenic Retroviridae Proteins, Tumor Necrosis Factor-alpha, Animals, Mice
Source:Journal of Neuroscience Research
ISSN:0360-4012
Publisher:Wiley (U.S.A.)
Volume:31
Number:4
Page Range:616-621
Date:1 April 1992
Official Publication:https://doi.org/10.1002/jnr.490310405
PubMed:View item in PubMed

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