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Lipopolysaccharide induces the rapid tyrosine phosphorylation of the mitogen-activated protein kinases erk-1 and p38 in cultured human vascular endothelial cells requiring the presence of soluble CD14

Item Type:Article
Title:Lipopolysaccharide induces the rapid tyrosine phosphorylation of the mitogen-activated protein kinases erk-1 and p38 in cultured human vascular endothelial cells requiring the presence of soluble CD14
Creators Name:Schumann, R.R. and Pfeil, D. and Lamping, N. and Kirschning, C. and Scherzinger, G. and Schlag, P.M. and Karawajew, L. and Herrmann, F.
Abstract:Human vascular endothelial cells (HUVECs), which do not display the lipopolysaccharide (LPS) receptor CD14, were examined for protein tyrosine phosphorylation after LPS stimulation in the presence and absence of soluble CD14 (sCD14). By phosphotyrosine Western blotting and immunocomplex kinase assays we show that LPS was capable of inducing in these cells rapid protein tyrosine phosphorylation and kinase activation of two members of the mitogen-activated protein kinase (MAPK) family erk-1 and the newly discovered p38, requiring the presence of sCD14. LPS-induced tyrosine phosphorylation of MAPK was associated with increased transcript- and surface protein expression of intracellular adhesion molecule-1 by HUVECs. MAPK phosphorylation and activation was induced by LPS in concentrations as little as 30 ng/mL and as early as 15 minutes after stimulation. Furthermore, tyrosine kinase inhibitors such as Genistein partially inhibited this effect. These results show that LPS triggers similar signaling events in both CD14+ myelo-monocytic cells and cells lacking the putative LPS-receptor CD14, suggesting the presence of a common, yet unidentified element in LPS-signaling in both cell types.
Keywords:Calcium-Calmodulin-Dependent Protein Kinases, CD14 Antigens, Cultured Cells, Lipopolysaccharides, Mitogen-Activated Protein Kinase 3, Mitogen-Activated Protein Kinases, p38 Mitogen-Activated Protein Kinases, Phosphorylation, Vascular Endothelium
Source:Blood
ISSN:0006-4971
Publisher:American Society of Hematology (U.S.A.)
Volume:87
Number:7
Page Range:2805-2814
Date:1 April 1996
Official Publication:http://bloodjournal.hematologylibrary.org/cgi/content/abstract/87/7/2805
PubMed:View item in PubMed

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