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A chimeric transactivator allows tetracycline-responsive gene expression in whole plants

Item Type:Article
Title:A chimeric transactivator allows tetracycline-responsive gene expression in whole plants
Creators Name:Weinmann, P. and Gossen, M. and Hillen, W. and Bujard, H. and Gatz, C.
Abstract:The chimeric transcriptional activator tTA, a fusion between the Tn10 encoded Tet repressor and the activation domain of the Herpes simplex virion protein VP16, was stably expressed in transgenic tobacco plants. It stimulates transcription of the beta-glucuronidase (gus) gene from an artificial promoter consisting of 7 tet operators and a TATA-box. Tetracycline, which interferes with binding of tTA to operator DNA, reduces gus expression over several orders of magnitude. This stringency of regulation suggests that the system can be used to construct transgenic plants encoding a potentially lethal gene product. Furthermore, the specific and fast inactivation of tTA allows study of the stability of RNAs and proteins.
Keywords:Antiporters, Base Sequence, Chimera, DNA, DNA Transposable Elements, Gene Expression Regulatio, Glucuronidase, Molecular Sequence Data, Genetically Modified Plants, Genetic Promoter Regions, Repressor Proteins, Simplexvirus, TATA Box, Tetracycline, Trans-Activators, Genetic Transformation, Viral Proteins
Source:Plant Journal
ISSN:0960-7412
Publisher:Wiley-Blackwell (U.K.)
Volume:5
Number:4
Page Range:559-569
Date:April 1994
PubMed:View item in PubMed

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