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| Item Type: | Article |
|---|---|
| Title: | Nitric oxide synthase isoforms I, III and protein kinase-C theta in skeletal muscle fibres of normal and streptozotocin-induced diabetic rats with and without Ginkgo biloba extract treatment |
| Creators Name: | Punkt, K., Zaitsev, S., Park, J.K., Wellner, M. and Buchwalow, I.B. |
| Abstract: | The expression of nitric oxide synthase (NOS) isoforms I, III and protein kinase-C{theta} (PKCθ) in rat vastus lateralis muscle was demonstrated immunohistochemically and then correlated to the physiological metabolic fibre types: SO (slow-oxidative), FOGI, FOGII (fast-oxidative glycolytic; I more glycolytic, II more oxidative), and FG (fast-glycolytic). NOS expression in muscles from different experimental groups (normal and diabetic rats, with and without Ginkgo biloba extract treatment) was assayed by Western blotting. Generally, NOS I and PKC{theta} were co-expressed in fibres with predominantly oxidative metabolism (SO, FOGII). This suggests an interplay of PKCθ and NOS I in nitric oxide production by oxidative fibres. NOS III was more highly expressed in fibres with predominantly glycolytic metabolism (FOGI, FG). A somewhat lower NOS I immunoreactivity was also found in NOS III positive fibres suggesting that NOS III and NOS I are co-expressed in these fibres. Western blotting revealed that NOS I as well as NOS III expression in the vastus lateralis muscle was down-regulated in diabetes and increased after Ginkgo biloba extract treatment. These effects may be associated with a diminished glucose uptake by myocytes of diabetic muscles and with an improved muscle function after Ginkgo biloba treatment. |
| Keywords: | Computer-Assisted Image Processing, Experimental Diabetes Mellitus, Fast-Twitch Muscle Fibers, Ginkgo Biloba, Immunohistochemistry, Isoenzymes, Muscle Fibers, Nitric Oxide Synthase, Nitric Oxide Synthase Type I, Nitric Oxide Synthase Type III, Oxidation-Reduction, Phytotherapy, Plant Extracts, Protein Kinase C, Slow-Twitch Muscle Fibers, Skeletal Muscle, Western Blotting, Wistar Rats, Animals, Rats |
| Source: | Histochemical Journal |
| ISSN: | 0018-2214 |
| Publisher: | Kluwer Academic Publishers |
| Volume: | 33 |
| Number: | 4 |
| Page Range: | 213-219 |
| Date: | 1 January 2001 |
| Official Publication: | https://doi.org/10.1023/A:1017994108174 |
| PubMed: | View item in PubMed |
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