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Apoptosis-induced cleavage of keratin 15 and keratin 17 in a human breast epithelial cell line

Item Type:Article
Title:Apoptosis-induced cleavage of keratin 15 and keratin 17 in a human breast epithelial cell line
Creators Name:Badock, V. and Steinhusen, U. and Bommert, K. and Wittmann-Liebold, B. and Otto, A.
Abstract:Keratin 15 (K15) and keratin 17 (K17) are intermediate filament (IF) type I proteins that are responsible for the mechanical integrity of epithelial cells. By analyzing the human breast epithelial cell line H184A1 before and after induction of apoptosis by high-resolution two-dimensional gel electrophoresis (2-DE) we identified the caspase-mediated cleavage of keratins 15 and 17. After induction of apoptosis three fragments of both K15 and K17 could be observed by 2 -DE. K15 and K17 proteolysis was observed during staurosporine-induced apoptosis and anoikis (anchorage-dependent apoptosis) as well and was shown to be caspase-dependent. By using mass spectrometry we could determine the caspase cleavage sites, one in K15 and two in K17. The sequence VEMD/A at the cleavage site located in the conserved linker region was found in K15 and K17. A further cleavage site was identified in the tail region of K17 with the recognition motif EVQD/G.
Keywords:Apoptosis, Epithelial cells, Keratin 15, Keratin 17, Two-dimensional gel electrophoresis, Caspases
Source:Cell Death and Differentiation
ISSN:1350-9047
Publisher:Nature Publishing Group (U.K.)
Volume:8
Number:3
Page Range:308-315
Date:1 March 2001
Official Publication:https://doi.org/10.1038/sj/cdd/4400812
PubMed:View item in PubMed

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