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| Item Type: | Article |
|---|---|
| Title: | Functional interference of Sp1 and NF-kappaB through the same DNA binding site |
| Creators Name: | Hirano, F., Tanaka, H., Hirano, Y., Hiramoto, M., Handa, H., Makino, I. and Scheidereit, C. |
| Abstract: | Gene activation by NF-kappaB/Rel transcription factors is modulated by synergistic or antagonistic interactions with other promoter-bound transcription factors. For example, Sp1 sites are often found in NF-kappaB-regulated genes, and Sp1 can activate certain promoters in synergism with NF-kappaB through nonoverlapping binding sites. Here we report that Sp1 acts directly through a subset of NF-kappaB binding sites. The DNA binding affinity of Sp1 to these NF-kappaB sites, as determined by their relative dissociation constants and their relative efficiencies as competitor DNAs or as binding site probes, is in the order of that for a consensus GC box Sp1 site. In contrast, NF-kappaB does not bind to a GC box Sp1 site. Sp1 can activate transcription through immunoglobulin kappa-chain enhancer or P-selectin promoter NF-kappaB sites. p50 homodimers replace Sp1 from the P-selectin promoter by binding site competition and thereby either inhibit basal Sp1-driven expression or, in concert with Bcl-3, stimulate expression. The interaction of Sp1 with NF-kappaB sites thus provides a means to keep an elevated basal expression of NF-kappaB-dependent genes in the absence of activated nuclear NF-kappaB/Rel. |
| Keywords: | Binding Sites, Cell Line, Consensus Sequence, DNA, Genetic Promoter Regions, HeLa Cells, NF-kappa B, NF-kappa B p50 Subunit, Oligodeoxyribonucleotides, Sp1 Transcription Factor, Transcription Factor RelA, Transcriptional Activation, Animals, Drosophila |
| Source: | Molecular and Cellular Biology |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Volume: | 18 |
| Number: | 3 |
| Page Range: | 1266-1274 |
| Date: | March 1998 |
| Official Publication: | http://mcb.asm.org/cgi/content/abstract/18/3/1266 |
| PubMed: | View item in PubMed |
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