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Hypo-osmotic cell swelling activates the p38 MAP kinase signalling cascade

Item Type:Article
Title:Hypo-osmotic cell swelling activates the p38 MAP kinase signalling cascade
Creators Name:Tilly, B.C. and Gaestel, M. and Engel, K. and Edixhoven, M.J. and de Jonge, H.R.
Abstract:Hypo-osmotic swelling of human Intestine 407 cells leads to a significant increase of intracellular MAPKAP-kinase 2 activity and Hsp27 phosphorylation. Pre-treatment of the cells with the p38 MAP kinase inhibitor SB-203580 blocks this activation, indicating that the hypotonicity-induced activation of MAPKAP kinase 2 is, similarly to that described for hyperosmotic treatment, the result of an activated p38 MAP kinase cascade. The activation of MAPKAP kinase 2 proceeds with kinetics similar to that of one of the first physiological responses of hypo-osmotic treatment, the opening of compensatory Cl- channels. However, inhibition of the p38 MAP kinase cascade does not block the osmo-sensitive anion efflux and, vice versa, activation of p38 MAP kinase by cytokines and anisomycin does not increase the efflux. These results indicate that the p38 MAP kinase cascade is not directly involved in Cl- channel activation but instead may play a role in subsequent cellular repair processes.
Keywords:Regulatory Volume Decrease, Chloride Channel, Reactivating Kinase, MAP Kinase, Heat Shock Protein, Cytokine
Source:FEBS Letters
Publisher:Elsevier (The Netherlands)
Page Range:133-136
Date:21 October 1996
Official Publication:https://doi.org/10.1016/0014-5793(96)01028-9
PubMed:View item in PubMed

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