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The receptor tyrosine kinase p185HER2 is expressed on a subset of B-lymphoid blasts from patients with acute lymphoblastic leukemia and chronic myelogenous leukemia

Item Type:Article
Title:The receptor tyrosine kinase p185HER2 is expressed on a subset of B-lymphoid blasts from patients with acute lymphoblastic leukemia and chronic myelogenous leukemia
Creators Name:Buehring, H.J. and Sures, I. and Jallal, B. and Weiss, F.U. and Busch, F.W. and Ludwig, W.D. and Handgretinger, R. and Waller, H.D. and Ullrich, A.
Abstract:The class I receptor tyrosine kinase (RTK) HER2 is an oncoprotein that is frequently involved in the pathogenesis of tumors of epithelial origin. Here we report mRNA expression in peripheral blood and bone marrow cells from healthy donors in hematopoietic cell lines and leukemic blasts from patients with acute lymphoblastic leukemia (ALL), acute myeloblastic leukemia (AML), chronic lymphoblastic leukemia (CLL), and chronic myeloid leukemia (CML). However, cell surface expression of HER2 protein (p185HER2) was found exclusively on a subset of leukemic cells of the B-lymphoblastic lineage. p185HER2 expression was found on blasts in 2 of 15 samples from infants, 9 of 19 samples from adult patients with C-ALL (CD19+CD10+), and 1 of 2 samples from patients with pro-B ALL (CD19+CD10-), whereas none of the leukemic cells from patients with AML (0/30), T-ALL (0/7), CLL (0/5) (CD19+CD5+), or CML in chronic and accelerated phase (0/5) or in blast crisis with myeloid differentiation (0/14) were positive for p185HER2. However, cells from 3 of 4 patients with CML in B-lymphoid blast crisis (CD19+CD10+) expressed high levels of p185HER2, which was also found on the surface of the CML-derived B-cell lines BV-173 and Nalm-1. Our study shows p185HER2 expression on malignant cells of hematopoietic origin for the first time. Aberrant expression of this oncogenic receptor tyrosine kinase in hematopoietic cell types may be an oncogenic event contributing to the development of a subset of B-lymphoblastic leukemias.
Keywords:3T3 Cells, Monoclonal Antibodies, CD34 Antigens, B-Lymphocyte Subsets, Blast Crisis, Bone Marrow, Cell Line, Cell Separation, Flow Cytometry, Fluorescent Antibody Technique, BCR-ABL Positive Chronic Myelogenous Leukemia, Phosphotyrosine, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Receptor Protein-Tyrosine Kinases, erbB-2 Receptor, Recombinant Proteins, Transfection, Cultured Tumor Cells, Biological Tumor Markers, Tyrosine, Animals, Mice
Source:Blood
ISSN:0006-4971
Publisher:American Society of Hematology (U.S.A.)
Volume:86
Number:5
Page Range:1916-1923
Date:1 September 1995
Official Publication:http://bloodjournal.hematologylibrary.org/cgi/content/abstract/86/5/1916
PubMed:View item in PubMed

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