Intranasal SARS-CoV-2 spike-based immunisation adjuvanted with polyethyleneimine elicits mucosal and systemic humoral responses in mice
Item Type: | Article |
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Title: | Intranasal SARS-CoV-2 spike-based immunisation adjuvanted with polyethyleneimine elicits mucosal and systemic humoral responses in mice |
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Creators Name: | Deimel, L.P. and Liu, X. and Gilbert-Jaramillo, J. and Liu, S. and James, W.S. and Sattentau, Q.J. |
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Abstract: | The SARS-CoV-2 pandemic continues despite the presence of effective vaccines, and novel vaccine approaches may help to reduce viral spread and associated COVID-19 disease. Current vaccine administration modalities are based on systemic needle-administered immunisation which may be suboptimal for mucosal pathogens. Here we demonstrate in a mouse model that small-volume intranasal administration of purified spike (S) protein in the adjuvant polyethylenemine (PEI) elicits robust antibody responses with modest systemic neutralisation activity. Further, we test a heterologous intranasal immunisation regimen, priming with S and boosting with RBD-Fc. Our data identify small volume PEI adjuvantation as a novel platform with potential for protective mucosal vaccine development. |
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Keywords: | SARS-CoV-2, COVID-19, Spike, RBD, Mucosal Adjuvant, Adaptive Immunity, Vaccine, Animals, Mice |
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Source: | Journal of Immunological Methods |
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ISSN: | 0022-1759 |
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Publisher: | Elsevier |
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Volume: | 511 |
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Page Range: | 113380 |
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Date: | December 2022 |
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Official Publication: | https://doi.org/10.1016/j.jim.2022.113380 |
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PubMed: | View item in PubMed |
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