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In vivo-mimicking microfluidic perfusion culture of pancreatic islet spheroids

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Item Type:Article
Title:In vivo-mimicking microfluidic perfusion culture of pancreatic islet spheroids
Creators Name:Jun, Y. and Lee, J.S. and Choi, S. and Yang, J.H. and Sander, M. and Chung, S. and Lee, S.H.
Abstract:Native pancreatic islets interact with neighboring cells by establishing three-dimensional (3D) structures, and are surrounded by perfusion at an interstitial flow level. However, flow effects are generally ignored in islet culture models, although cell perfusion is known to improve the cell microenvironment and to mimic in vivo physiology better than static culture systems. Here, we have developed functional islet spheroids using a microfluidic chip that mimics interstitial flow conditions with reduced shear cell damage. Dynamic culture, compared to static culture, enhanced islet health and maintenance of islet endothelial cells, reconstituting the main component of islet extracellular matrix within spheroids. Optimized flow condition allowed localization of secreted soluble factors near spheroids, facilitating diffusion-mediated paracrine interactions within islets, and enabled long-term maintenance of islet morphology and function for a month. The proposed model can aid islet preconditioning before transplantation and has potential applications as an in vitro model for diabetic drug testing.
Source:Science Advances
ISSN:2375-2548
Publisher:American Association for the Advancement of Science
Volume:5
Number:11
Page Range:eaax4520
Date:1 November 2019
Official Publication:https://doi.org/10.1126/sciadv.aax4520
PubMed:View item in PubMed

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