Insights into pancreatic β cell energy metabolism using rodent β cell models

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Item Type:	Article
Title:	Insights into pancreatic β cell energy metabolism using rodent β cell models
Creators Name:	Morten, K.J., Potter, M., Badder, L., Sivathondan, P., Dragovic, R., Neumann, A., Gavin, J., Shrestha, R., Reilly, S., Phadwal, K., Lodge, T.A., Borzychowski, A., Cookson, S., Mitchell, C., Morovat, A., Simon, A.K., Uusimaa, J., Hynes, J. and Poulton, J.
Abstract:	Mitochondrial diabetes is primarily caused by β-cell failure, a cell type whose unique properties are important in pathogenesis. By reducing glucose, we induced energetic stress in two rodent β-cell models to assess effects on cellular function. Culturing rat insulin-secreting INS-1 cells in low glucose conditions caused a rapid reduction in whole cell respiration, associated with elevated mitochondrial reactive oxygen species production, and an altered glucose-stimulated insulin secretion profile. Prolonged exposure to reduced glucose directly impaired mitochondrial function and reduced autophagy. Insulinoma cell lines have a very different bioenergetic profile to many other cell lines and provide a useful model of mechanisms affecting β-cell mitochondrial function.
Keywords:	Beta-Cell, Oxidative Phosphorylation, Reactive Oxygen Species, Superoxide, Mitochondria, Insulin Secretion
Source:	Wellcome Open Research
ISSN:	2398-502X
Publisher:	Wellcome
Volume:	2
Page Range:	14
Date:	25 September 2019
Official Publication:	https://doi.org/10.12688/wellcomeopenres.10535.1
PubMed:	View item in PubMed

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