Dual RNA-seq of pathogen and host

Item Type:	Review
Title:	Dual RNA-seq of pathogen and host
Creators Name:	Westermann, A.J. and Gorski, S.A. and Vogel, J.
Abstract:	A comprehensive understanding of host–pathogen interactions requires a knowledge of the associated gene expression changes in both the pathogen and the host. Traditional, probe-dependent approaches using microarrays or reverse transcription PCR typically require the pathogen and host cells to be physically separated before gene expression analysis. However, the development of the probe-independent RNA sequencing (RNA-seq) approach has begun to revolutionize transcriptomics. Here, we assess the feasibility of taking transcriptomics one step further by performing 'dual RNA-seq', in which gene expression changes in both the pathogen and the host are analysed simultaneously.
Keywords:	Animal Disease Models, Bacterial Infections, Computational Biology, Gene Expression Profiling, Host-Pathogen Interactions, Mycoses, Plant Diseases, Protozoan Infections, RNA Sequence Analysis, Animals
Source:	Nature Reviews Microbiology
ISSN:	1740-1526
Publisher:	Nature Publishing Group
Volume:	10
Number:	9
Page Range:	618-630
Date:	September 2012
Official Publication:	https://doi.org/10.1038/nrmicro2852
PubMed:	View item in PubMed

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