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| Item Type: | Article |
|---|---|
| Title: | Single-cell RNA-seq: advances and future challenges |
| Creators Name: | Saliba, A.E. and Westermann, A.J. and Gorski, S.A. and Vogel, J. |
| Abstract: | Phenotypically identical cells can dramatically vary with respect to behavior during their lifespan and this variation is reflected in their molecular composition such as the transcriptomic landscape. Single-cell transcriptomics using next-generation transcript sequencing (RNA-seq) is now emerging as a powerful tool to profile cell-to-cell variability on a genomic scale. Its application has already greatly impacted our conceptual understanding of diverse biological processes with broad implications for both basic and clinical research. Different single-cell RNA-seq protocols have been introduced and are reviewed here-each one with its own strengths and current limitations. We further provide an overview of the biological questions single-cell RNA-seq has been used to address, the major findings obtained from such studies, and current challenges and expected future developments in this booming field. |
| Keywords: | Cell Separation, Gene Expression Profiling, High-Throughput Nucleotide Sequencing, RNA Sequence Analysis, Single-Cell Analysis |
| Source: | Nucleic Acids Research |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Volume: | 42 |
| Number: | 14 |
| Page Range: | 8845-8860 |
| Date: | 18 August 2014 |
| Official Publication: | https://doi.org/10.1093/nar/gku555 |
| PubMed: | View item in PubMed |
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