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Suicide nanoplasmids coding for ribosome-inactivating proteins

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Item Type:Article
Title:Suicide nanoplasmids coding for ribosome-inactivating proteins
Creators Name:Mitdank, H. and Tröger, M. and Sonntag, A. and Shirazi, N.A. and Woith, E. and Fuchs, H. and Kobelt, D. and Walther, W. and Weng, A.
Abstract:Conventional eukaryotic expression plasmids contain a DNA backbone that is dispensable for the cellular expression of the transgene. In order to reduce the vector size, minicircle DNA technology was introduced. A drawback of the minicircle technology are considerable production costs. Nanoplasmids are a relatively new class of mini-DNA constructs that are of tremendous potential for pharmaceutical applications. In this study we have designed novel suicide nanoplasmid constructs coding for plant derived ribosome-inactivating proteins. The suicide-nanoplasmids were formulated with a targeted K(16)-lysine domain, analyzed for size, and characterized by electron microscopy. The anti-proliferative activity of the suicide-nanoplasmids was investigated in vitro by real time microscopy and the expression kinetic was determined using an enhanced green fluorescent protein nanoplasmid variant. In an aggressive in vivo neuroblastoma tumor model, treated mice showed a reduced tumor growth whereby the therapy was well tolerated.
Keywords:Suicide Gens, Toxins, Anti-Tumor, Nanoplasmid, Animals, Mice
Source:European Journal of Pharmaceutical Sciences
ISSN:0928-0987
Publisher:Elsevier
Volume:170
Page Range:106107
Date:1 March 2022
Official Publication:https://doi.org/10.1016/j.ejps.2021.106107
PubMed:View item in PubMed

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