Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

WASp controls oriented migration of endothelial cells to achieve functional vascular patterning

[img]
Preview
PDF (Original Article) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
14MB
[img]
Preview
PDF (Supplementary Information) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
10MB

Item Type:Article
Title:WASp controls oriented migration of endothelial cells to achieve functional vascular patterning
Creators Name:Rosa, A. and Giese, W. and Meier, K. and Alt, S. and Klaus-Bergmann, A. and Edgar, L.T. and Bartels, E. and Collins, R. and Szymborska, A. and Coxam, B. and Bernabeu, M.O. and Gerhardt, H.
Abstract:Endothelial cell migration and proliferation are essential for the establishment of a hierarchical organization of blood vessels and optimal distribution of blood. However, how these cellular processes are coordinated remains unknown. Here, using the zebrafish trunk vasculature we show that in future veins endothelial cells proliferate more than in future arteries and migrate preferentially towards neighboring arteries. In future arteries endothelial cells show a biphasic migration profile. During sprouting cells move away from the dorsal aorta, during remodelling cells stop or move towards the feeding aorta. The final morphology of blood vessels is thus established by local proliferation and oriented cell migration to and from neighboring vessels. Additionally, we identify WASp to be essential for this differential migration. Loss of WASp leads to irregular distribution of endothelial cells, substantially enlarged veins and persistent arteriovenous shunting. Mechanistically, we report that WASp drives the assembly of junctional associated actin filaments and is required for junctional expression of PECAM-1. Together, our data identify that functional vascular patterning in the zebrafish trunk utilizes differential cell movement regulated by junctional actin, and that interruption of differential migration may represent a pathomechanism in vascular malformations.
Keywords:Migration, Proliferation, WASp, F-actin, Vessel Remodelling, Animals, Zebrafish
Source:Development
ISSN:0950-1991
Publisher:Company of Biologists
Volume:149
Number:3
Page Range:dev200195
Date:February 2022
Official Publication:https://doi.org/10.1242/dev.200195
PubMed:View item in PubMed
Related to:
URLURL Type
https://edoc.mdc-berlin.de/19385/Preprint version

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library