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3D super-resolution fluorescence microscopy maps the variable molecular architecture of the nuclear pore complex

Item Type:Article
Title:3D super-resolution fluorescence microscopy maps the variable molecular architecture of the nuclear pore complex
Creators Name:Sabinina, V.J. and Hossain, M.J. and Hériché, J.K. and Hoess, P. and Nijmeijer, B. and Mosalaganti, S. and Kueblbeck, M. and Callegari, A. and Szymborska, A. and Beck, M. and Ries, J. and Ellenberg, J.
Abstract:Nuclear pore complexes (NPCs) are large macromolecular machines that mediate the traffic between the nucleus and the cytoplasm. In vertebrates, each NPC consists of ∼1000 proteins, termed nucleoporins, and has a mass of over 100 MDa. While a pseudo-atomic static model of the central scaffold of the NPC has recently been assembled by integrating data from isolated proteins and complexes, many structural components still remain elusive due to the enormous size and flexibility of the NPC. Here, we explored the power of 3D super-resolution microscopy combined with computational classification and averaging to explore the 3D structure of the NPC in single human cells. We show that this approach can build the first integrated 3D structural map containing both central as well as peripheral NPC subunits with molecular specificity and nanoscale resolution. Our unbiased classification of over ten thousand individual NPCs indicates that the nuclear ring and the nuclear basket can adopt different conformations. Our approach opens up the exciting possibility to relate different structural states of the NPC to function in situ.
Keywords:Cell Nucleus, Cytoplasm, Fluorescence Microscopy, Nuclear Pore, Nuclear Pore Complex Proteins, Animals
Source:Molecular Biology of the Cell
ISSN:1059-1524
Publisher:American Society for Cell Biology
Volume:32
Number:17
Page Range:1523-1533
Date:15 August 2021
Official Publication:https://doi.org/10.1091/mbc.E20-11-0728
PubMed:View item in PubMed
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https://edoc.mdc-berlin.de/19751/Preprint version

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