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A community-driven roadmap to advance research on translated open reading frames detected by Ribo-seq

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Item Type:Preprint
Title:A community-driven roadmap to advance research on translated open reading frames detected by Ribo-seq
Creators Name:Mudge, J.M. and Ruiz-Orera, J. and Prensner, J.R. and Brunet, M.A. and Gonzalez, J.M. and Magrane, M. and Martinez, T. and Schulz, J.F. and Yang, Y.T. and Albà, M.M. and Baranov, P.V. and Bazzini, A. and Bruford, E. and Martin, M.J. and Carvunis, A.R. and Chen, J. and Couso, J.P. and Flicek, P. and Frankish, A. and Gerstein, M. and Hubner, N. and Ingolia, N.T. and Menschaert, G. and Ohler, U. and Roucou, X. and Saghatelian, A. and Weissman, J. and van Heesch, S.
Abstract:Ribosome profiling (Ribo-seq) has catalyzed a paradigm shift in our understanding of the translational ‘vocabulary’ of the human genome, discovering thousands of translated open reading frames (ORFs) within long non-coding RNAs and presumed untranslated regions of protein-coding genes. However, reference gene annotation projects have been circumspect in their incorporation of these ORFs due to uncertainties about their experimental reproducibility and physiological roles. Yet, it is indisputable that certain Ribo-seq ORFs make stable proteins, others mediate gene regulation, and many have medical implications. Ultimately, the absence of standardized ORF annotation has created a circular problem: while Ribo-seq ORFs remain unannotated by reference biological databases, this lack of characterisation will thwart research efforts examining their roles. Here, we outline the initial stages of a community-led effort supported by GENCODE / Ensembl, HGNC and UniProt to produce a consolidated catalog of human Ribo-seq ORFs.
Publisher:Cold Spring Harbor Laboratory Press
Article Number:2021.06.10.447896
Date:10 June 2021
Official Publication:https://doi.org/10.1101/2021.06.10.447896
Related to:
https://edoc.mdc-berlin.de/21818/Final version

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