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| Item Type: | Article |
|---|---|
| Title: | Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status |
| Creators Name: | Coscia, F. and Watters, K.M. and Curtis, M. and Eckert, M.A. and Chiang, C.Y. and Tyanova, S. and Montag, A. and Lastra, R.R. and Lengyel, E. and Mann, M. |
| Abstract: | A cell line representative of human high-grade serous ovarian cancer (HGSOC) should not only resemble its tumour of origin at the molecular level, but also demonstrate functional utility in pre-clinical investigations. Here, we report the integrated proteomic analysis of 26 ovarian cancer cell lines, HGSOC tumours, immortalized ovarian surface epithelial cells and fallopian tube epithelial cells via a single-run mass spectrometric workflow. The in-depth quantification of >10,000 proteins results in three distinct cell line categories: epithelial (group I), clear cell (group II) and mesenchymal (group III). We identify a 67-protein cell line signature, which separates our entire proteomic data set, as well as a confirmatory publicly available CPTAC/TCGA tumour proteome data set, into a predominantly epithelial and mesenchymal HGSOC tumour cluster. This proteomics-based epithelial/mesenchymal stratification of cell lines and human tumours indicates a possible origin of HGSOC either from the fallopian tube or from the ovarian surface epithelium. |
| Keywords: | Datasets as Topic, Epithelial Cells, Fallopian Tubes, Gene Expression Profiling, Mass Spectrometry, Neoplasm Grading, Ovarian Neoplasms, Ovary, Primary Cell Culture, Proteomics, Transcriptome, Tumor Cell Line |
| Source: | Nature Communications |
| ISSN: | 2041-1723 |
| Publisher: | Nature Publishing Group |
| Volume: | 7 |
| Page Range: | 12645 |
| Date: | 26 August 2016 |
| Official Publication: | https://doi.org/10.1038/ncomms12645 |
| PubMed: | View item in PubMed |
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