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Dual-view light-sheet imaging through a tilted glass interface using a deformable mirror

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Item Type:Article
Title:Dual-view light-sheet imaging through a tilted glass interface using a deformable mirror
Creators Name:Vladimirov, N., Preusser, F., Wisniewski, J., Yaniv, Z., Desai, R.A., Woehler, A. and Preibisch, S.
Abstract:Light-sheet microscopy has become indispensable for imaging developing organisms, and imaging from multiple directions (views) is essential to improve its spatial resolution. We combine multi-view light-sheet microscopy with microfluidics using adaptive optics (deformable mirror) which corrects aberrations introduced by the 45°-tilted glass coverslip. The optimal shape of the deformable mirror is computed by an iterative algorithm that optimizes the point-spread function in two orthogonal views. Simultaneous correction in two optical arms is achieved via a knife-edge mirror that splits the excitation path and combines the detection paths. Our design allows multi-view light-sheet microscopy with microfluidic devices for precisely controlled experiments and high-content screening.
Keywords:Animals, Caenorhabditis elegans
Source:Biomedical Optics Express
ISSN:2156-7085
Publisher:Optical Society of America
Volume:12
Number:4
Page Range:2186-2203
Date:1 April 2021
Official Publication:https://doi.org/10.1364/BOE.416737
PubMed:View item in PubMed

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