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| Item Type: | Preprint |
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| Title: | Mitochondrial variant enrichment from high-throughput single-cell RNA-seq resolves clonal populations |
| Creators Name: | Miller, T.E. and Lareau, C.A. and Verga, J.A. and Ssozi, D. and Ludwig, L.S. and El Farran, C. and Griffin, G.K. and Lane, A.A. and Bernstein, B.E. and Sankaran, V.G. and van Galen, P. |
| Abstract: | Reconstructing lineage relationships in complex tissues can reveal mechanisms underlying development and disease. Recent methods combine single-cell transcriptomics with mitochondrial DNA variant detection to establish lineage relationships in primary human cells, but are not scalable to interrogate complex tissues. To overcome this limitation, here we develop a technology for high-confidence detection of mitochondrial mutations from high-throughput single-cell RNA-sequencing. We use the new method to identify skewed immune cell expansions in primary human clonal hematopoiesis. |
| Source: | bioRxiv |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Article Number: | 2021.03.08.434450 |
| Date: | 9 March 2021 |
| Official Publication: | https://doi.org/10.1101/2021.03.08.434450 |
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