SEMITONES: Single-cEll Marker IdentificaTiON by Enrichment Scoring

Preview

PDF (Preprint) - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
3MB

Other (Supplementary Material)
4MB

Item Type:

Preprint

Title:

SEMITONES: Single-cEll Marker IdentificaTiON by Enrichment Scoring

Creators Name:

Vlot, A.H.C. and Maghsudi, S. and Ohler, U.

Abstract:

Identification of markers is an essential step in single-cell analytic. Current marker identification strategies typically rely on cluster assignments of cells. Cluster assignment, in particular of development data, is non-trivial, potentially arbitrary and commonly relies on prior knowledge. Yet, cluster uncertainty is not commonly taken into account. In response, we present SEMITONES, a principled method for cluster-free marker identification. We showcase its application on healthy haematopoiesis data as 1) a robust alternative to highly variable gene selection, 2) for marker gene and regulatory region identification, and 3) for the construction of co-enrichment networks that reveal regulators of cell identity.

Source:

bioRxiv

Publisher:

Cold Spring Harbor Laboratory Press

Article Number:

2020.11.17.386664

Date:

18 November 2020

Official Publication:

https://doi.org/10.1101/2020.11.17.386664

Related to: