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SEMITONES: Single-cEll Marker IdentificaTiON by Enrichment Scoring

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Item Type:Preprint
Title:SEMITONES: Single-cEll Marker IdentificaTiON by Enrichment Scoring
Creators Name:Vlot, A.H.C. and Maghsudi, S. and Ohler, U.
Abstract:Identification of markers is an essential step in single-cell analytic. Current marker identification strategies typically rely on cluster assignments of cells. Cluster assignment, in particular of development data, is non-trivial, potentially arbitrary and commonly relies on prior knowledge. Yet, cluster uncertainty is not commonly taken into account. In response, we present SEMITONES, a principled method for cluster-free marker identification. We showcase its application on healthy haematopoiesis data as 1) a robust alternative to highly variable gene selection, 2) for marker gene and regulatory region identification, and 3) for the construction of co-enrichment networks that reveal regulators of cell identity.
Source:bioRxiv
Publisher:Cold Spring Harbor Laboratory Press
Article Number:2020.11.17.386664
Date:18 November 2020
Official Publication:https://doi.org/10.1101/2020.11.17.386664
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https://edoc.mdc-berlin.de/21972/Final version

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