Helmholtz Gemeinschaft


Single-nuclei RNA-sequencing of plants

Item Type:Preprint
Title:Single-nuclei RNA-sequencing of plants
Creators Name:Sunaga-Franze, D.Y. and Muino, J.M. and Braeuning, C. and Xu, X. and Zong, M. and Smaczniak, C. and Yan, W. and Fischer, C. and Vidal, R. and Kliem, M. and Kaufmann, K. and Sauer, S.
Abstract:Single-cell genomics has slowly advanced in plant research. Here, we introduce a generic procedure for plant nuclei isolation and nanowell-based library preparation for short-read sequencing. This plant-nuclei sequencing (PN-seq) method allows for analyzing several thousands of genes in thousands of individual plant cells. In this way, we expand the toolset for single-cell genomics in the field of plant biology to generate plant transcriptome atlases in development and environmental response.
Publisher:Cold Spring Harbor Laboratory Press
Article Number:2020.11.14.382812
Date:16 November 2020
Official Publication:https://doi.org/10.1101/2020.11.14.382812

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