Effect of vitamin D supplementation on N-glycan branching and cellular immunophenotypes in MS

Bäcker-Koduah, P.; Infante-Duarte, C.; Ivaldi, F.; Uccelli, A.; Bellmann-Strobl, J.; Wernecke, K.D.; Sy, M.; Demetriou, M.; Dörr, J.; Paul, F.; Brandt, A.U.

Effect of vitamin D supplementation on N-glycan branching and cellular immunophenotypes in MS

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Item Type:	Article
Title:	Effect of vitamin D supplementation on N-glycan branching and cellular immunophenotypes in MS
Creators Name:	Bäcker-Koduah, P., Infante-Duarte, C., Ivaldi, F., Uccelli, A., Bellmann-Strobl, J., Wernecke, K.D., Sy, M., Demetriou, M., Dörr, J., Paul, F. and Brandt, A.U.
Abstract:	OBJECTIVE: To investigate the effect of cholecalciferol (vitamin D3) supplementation on peripheral immune cell frequency and N-glycan branching in patients with relapsing-remitting multiple sclerosis (RRMS). METHODS: Exploratory analysis of high-dose (20 400 IU) and low-dose (400 IU) vitamin D3 supplementation taken every other day of an 18-month randomized controlled clinical trial including 38 RRMS patients on stable immunomodulatory therapy (NCT01440062). We investigated cholecalciferol treatment effects on N-glycan branching using L-PHA stain (phaseolus vulgaris leukoagglutinin) at 6 months and frequencies of T-, B-, and NK-cell subpopulations at 12 months with flow cytometry. RESULTS: High-dose supplementation did not change CD3+ T cell subsets, CD19+ B cells subsets, and NK cells frequencies, except for CD8+ T regulatory cells, which were reduced in the low-dose arm compared to the high-dose arm at 12 months. High-dose supplementation decreased N-glycan branching on T and NK cells, measured as L-PHA mean fluorescence intensity (MFI). A reduction of N-glycan branching in B cells was not significant. In contrast, low-dose supplementation did not affect N-glycan branching. Changes in N-glycan branching did not correlate with cell frequencies. INTERPRETATION: Immunomodulatory effect of vitamin D may involve regulation of N-glycan branching in vivo. Vitamin D3 supplementation did at large not affect the frequencies of peripheral immune cells.
Keywords:	B-Lymphocyte Subsets, Cholecalciferol, Dietary Supplements, Immunologic Factors, Natural Killer Cells, Polysaccharides, Relapsing-Remitting Multiple Sclerosis, T-Lymphocyte Subsets, Treatment Outcome
Source:	Annals of Clinical and Translational Neurology
ISSN:	2328-9503
Publisher:	Wiley
Volume:	7
Number:	9
Page Range:	1628-1641
Date:	September 2020
Official Publication:	https://doi.org/10.1002/acn3.51148
PubMed:	View item in PubMed

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