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Dual-mode detection of bacterial 16S ribosomal RNA in tissues

Item Type:Article
Title:Dual-mode detection of bacterial 16S ribosomal RNA in tissues
Creators Name:Xu, S. and Zhang, Q. and Han, X. and Wang, Y. and Wang, X. and Nazaré, M. and Jiang, J.D. and Hu, H.Y.
Abstract:The specific detection of pathogens has long been recognized as a vital strategy for controlling bacterial infections. Herein, a novel hydrophilic aromatic-imide-based thermally activated delayed fluorescence (TADF) probe, AI-Cz-Neo, is designed and synthesized by conjugation of a TADF emitter with a bacterial 16S ribosomal RNA-targeted moiety, neomycin. Biological data showed for the first time that AI-Cz-Neo could be successfully applied for the dual-mode detection of bacterial 16S rRNA using confocal fluorescence imaging and time-resolved fluorescence imaging (TRFI) in both cells and tissues. These findings greatly expand the application of TADF fluorophores in time-resolved biological imaging and provide a promising strategy for the precise and reliable diagnosis of bacterial infections based on the dual-mode imaging of bacterial 16S rRNA by fluorescence intensity and fluorescence lifetime.
Keywords:16S RibosomalFluorescent Dyes, Hydrophobic and Hydrophilic Interactions, Neomycin, Optical Imaging, 16S Ribosomal RNA
Source:ACS Sensors
Publisher:American Chemical Society
Page Range:1650-1656
Date:26 June 2020
Official Publication:https://doi.org/10.1021/acssensors.0c00252
PubMed:View item in PubMed

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