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| Item Type: | Preprint | ||||
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| Title: | UbiFast, a rapid and deep-scale ubiquitylation profiling approach for biology and translational research | ||||
| Creators Name: | Udeshi, N.D. and Mani, D.C. and Satpathy, S. and Fereshetian, S. and Gasser, J.A. and Svinkina, T. and Ebert, B.L. and Mertins, P. and Carr, S.A. | ||||
| Abstract: | Protein ubiquitylation is involved in a plethora of cellular processes. Defects in the ubiquitin system are at the root of many acquired and hereditary diseases. While antibodies directed at ubiquitin remnants (K-ε-GG) have improved the ability to monitor ubiquitylation using mass spectrometry, methods for highly-multiplexed measurement of ubiquitylation in tissues and primary cells using sub-milligram amounts of sample remains a challenge. Here we present a highly-sensitive, rapid and multiplexed protocol for quantifying ∼10,000 ubiquitylation sites from as little as 500 ug peptide per sample from cells or tissue in a TMT10 plex in ca. 5 hr. High-field Asymmetric Ion Mobility Spectrometry (FAIMS) is used to improve quantitative accuracy for posttranslational modification analysis. We use the approach to rediscover substrates of the E3 ligase targeting drug lenalidomide and to identify proteins modulated by ubiquitylation in models of basal and luminal human breast cancer. The sensitivity and speed of the UbiFast method makes it suitable for large-scale studies in primary tissue samples. | ||||
| Source: | bioRxiv | ||||
| Publisher: | Cold Spring Harbor Laboratory Press | ||||
| Article Number: | 785378 | ||||
| Date: | 27 September 2019 | ||||
| Official Publication: | https://doi.org/10.1101/785378 | ||||
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