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RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses

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Item Type:Preprint
Title:RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses
Creators Name:Brockmann, M.M. and Maglione, M. and Willmes, C.G. and Stumpf, A. and Bouazza, B.A. and Velasquez, L.M. and Grauel, M.K. and Beed, P. and Sigrist, S.J. and Rosenmund, C. and Schmitz, D.
Abstract:All synapses require fusion-competent vesicles and coordinated Ca(2+)-secretion coupling for neurotransmission, yet functional and anatomical properties show a high diversity across different synapse types. We show here that the presynaptic protein RIM-BP2 has diversified functions in neurotransmitter release at different central mammalian synapses and thus contributes to synaptic diversity. At hippocampal pyramidal CA3-CA1 synapses, RIM-BP2 loss has a mild effect on neurotransmitter release, by only regulating Ca(2+)-secretion coupling. However, at hippocampal mossy fiber synapses RIM-BP2 has a strong impact on neurotransmitter release by promoting vesicle docking/priming via recruitment of Munc13-1. In wild type mossy fiber synapses, the distance between RIM-BP2 clusters and Munc13-1 clusters is larger than in hippocampal pyramidal CA3-CA1 synapses, suggesting that spatial organization may dictate the role a protein plays in synaptic transmission and that differences in active zone architecture is a major determinant factor in the functional diversity of synapses.
Source:bioRxiv
Publisher:Cold Spring Harbor Laboratory Press (U.S.A.)
Article Number:469494
Date:13 November 2018
Official Publication:https://doi.org/10.1101/469494
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https://edoc.mdc-berlin.de/18443/Final version

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