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Imaging glioma progression by intravital microscopy

Item Type:Article
Title:Imaging glioma progression by intravital microscopy
Creators Name:Stanchi, F. and Matsumoto, K. and Gerhardt, H.
Abstract:We describe here a method for generating mouse orthotopic gliomas in order to follow their progression over time by multi-photon laser scanning microscopy. After craniotomy of the parietal bone, glioma cells are implanted in the brain cortex and a glass window is cemented atop, allowing chronical imaging of the tumor. The expression of different fluorescent proteins in tumor cells and in specific cell types of a number of currently available transgenic mouse strains allows obtaining multicolor 3D images of the tumor over time. This technique is suitable both to evaluate the effect of pharmacological treatments and to unravel basic mechanisms of tumor-host interactions.
Keywords:Intravital Imaging, Multi-Photon, Laser Scanning Microscopy, Tumor Model, Glioma, Animals, Mice
Source:Methods in Molecular Biology
Series Name:Methods in Molecular Biology
Title of Book:Metabolic Signaling
ISSN:1064-3745
ISBN:978-1-4939-8768-9
Publisher:Springer / Humana Press (U.S.A.)
Volume:1862
Page Range:227-243
Date:2019
Official Publication:https://doi.org/10.1007/978-1-4939-8769-6_16
PubMed:View item in PubMed

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