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NanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes

Item Type:Article
Title:NanoSIMS imaging reveals unexpected heterogeneity in nutrient uptake by brown adipocytes
Creators Name:He, C. and Hu, X. and Weston, T.A. and Jung, R.S. and Heizer, P. and Tu, Y. and Ellison, R. and Matsumoto, K. and Gerhardt, H. and Tontonoz, P. and Fong, L.G. and Young, S.G. and Jiang, H.
Abstract:Heterogeneity in the metabolic properties of adipocytes in white adipose tissue has been well documented. We sought to investigate metabolic heterogeneity in adipocytes of brown adipose tissue (BAT), focusing on heterogeneity in nutrient uptake. To explore the possibility of metabolic heterogeneity in brown adipocytes, we used nanoscale secondary ion mass spectrometry (NanoSIMS) to quantify uptake of lipids in adipocytes interscapular BAT and perivascular adipose tissue (PVAT) after an intravenous injection of triglyceride-rich lipoproteins (TRLs) containing [2H]triglycerides (2H-TRLs). The uptake of deuterated lipids into brown adipocytes was quantified by NanoSIMS. We also examined 13C enrichment in brown adipocytes after administering [13C]glucose or 13C-labeled mixed fatty acids by gastric gavage. The uptake of 2H-TRLs-derived lipids into brown adipocytes was heterogeneous, with 2H enrichment in adjacent adipocytes varying by more than fourfold. We also observed substantial heterogeneity in 13C enrichment in adjacent brown adipocytes after administering [13C]glucose or [13C]fatty acids by gastric gavage. The uptake of nutrients by adjacent brown adipocytes within a single depot is variable, suggesting that there is heterogeneity in the metabolic properties of brown adipocytes.
Keywords:Brown Adipose Tissue, White Adipose Tissue, NanoSIMS Imaging, Nutrient Uptake, Fatty Acids, Animals, Mice
Source:Biochemical and Biophysical Research Communications
ISSN:0006-291X
Publisher:Academic Press (U.S.A.)
Volume:504
Number:4
Page Range:899-902
Date:12 October 2018
Official Publication:https://doi.org/10.1016/j.bbrc.2018.09.051
PubMed:View item in PubMed

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