Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Regulation of spatial and temporal gene expression in an animal germline

Item Type:Preprint
Title:Regulation of spatial and temporal gene expression in an animal germline
Creators Name:Diag, A. and Schilling, M. and Klironomos, F. and Ayoub, S. and Rajewsky, N.
Abstract:In animal germlines, regulation of cell proliferation and differentiation is particularly important but poorly understood. Here, using a cryo-cut approach, we mapped RNA expression along the Caenorhabditis elegans germline and, using mutants, dissected gene regulatory mechanisms that control spatio-temporal expression. We detected, at near single-cell resolution, > 10,000 mRNAs, > 300 miRNAs and numerous novel miRNAs. Most RNAs were organized in distinct spatial patterns. Germline-specific miRNAs and their targets were co-localized. Moreover, we observed differential 3' UTR isoform usage for hundreds of mRNAs. In tumorous gld-2 gld-1 mutants, gene expression was strongly perturbed. In particular, differential 3' UTR usage was significantly impaired. We propose that PIE-1, a transcriptional repressor, functions to maintain spatial gene expression. Our data also suggest that cpsf-4 and fipp-1 control differential 3' UTR usage for hundreds of genes. Finally, we constructed a "virtual gonad" enabling "virtual in situ hybridizations" and access to all data (https://shiny.mdc-berlin.de/spacegerm/).
Source:bioRxiv
Publisher:Cold Spring Harbor Laboratory (U.S.A.)
Article Number:348425
Date:15 June 2018
Official Publication:https://doi.org/10.1101/348425

Repository Staff Only: item control page

Open Access
MDC Library