Helmholtz Gemeinschaft

Search
Browse
Statistics
Feeds

Purification of cross-linked RNA-protein complexes by phenol-toluol extraction

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
9MB
Item Type:Preprint
Title:Purification of cross-linked RNA-protein complexes by phenol-toluol extraction
Creators Name:Urdaneta, E.C. and Vieira-Vieira, C.H. and Hick, T. and Wessels, H.H. and Figini, D. and Moschall, R. and Medenbach, J. and Ohler, U. and Granneman, S. and Selbach, M. and Beckmann, B.M.
Abstract:Recent methodological advances allowed the identification of an increasing number of RNA-binding proteins (RBPs) and their RNA-binding sites. Most of those methods rely, however, on capturing proteins associated to polyadenylated RNAs which neglects RBPs bound to non-adenylate RNA classes (tRNA, rRNA, pre-mRNA) as well as the vast majority of species that lack poly-A tails in their mRNAs (including all archea and bacteria). To overcome these limitations, we have developed a novel protocol, Phenol Toluol extraction (PTex), that does not rely on a specific RNA sequence or motif for isolation of cross-linked ribonucleoproteins (RNPs), but rather purifies them based entirely on their physicochemical properties. PTex captures RBPs that bind to RNA as short as 30 nt, RNPs directly from animal tissue and can be used to simplify complex workflows such as PAR-CLIP. Finally, we provide a first global RNA-bound proteome of human HEK293 cells and Salmonella Typhimurium as a bacterial species.
Source:bioRxiv
Publisher:Cold Spring Harbor Laboratory (U.S.A.)
Article Number:333385
Date:30 May 2018
Official Publication:https://doi.org/10.1101/333385
Related to:
URLURL Type
https://edoc.mdc-berlin.de/18082/Final version

Repository Staff Only: item control page

Downloads

Downloads per month over past year

Open Access
MDC Library